ABSTRACT
Evidence from several lines of investigations suggests that Toll-like receptor 4 [TLR4] is involved in atherosclerosis as a bridge between innate and acquired immunity. Percutaneous coronary intervention [PCI] can trigger inflammation through activation of human TLR4 [hTLR4] on monocytes. Hydrocortisone as an anti-inflammatory and immuno-suppressant agent has multiple mechanisms of action. In this study, we aimed at assessing the effects of hydrocortisone on monocyte expression and activity of hTLR4 in patients underwent PCI. Blood samples were taken from a total of 71 patients with chronic stable angina who were scheduled for a PCI, before the intervention. Thirty patients received 100 mg hydrocortisone prior to the procedure. Control group was composed of 41 patients underwent PCI without receiving hydrocortisone. Blood collection was repeated 2 and 4 h after PCI. The expression of hTLR4 on the surface of CD14[+] monocytes and the serum levels of TNF- alpha and IL-1 beta were measured using flowcytometry and Sandwich ELISA. Compared with controls, hydrocortisone significantly reduced monocyte expression of hTLR4 in test group [P<0.01]. In addition, it had a significant effect on reduction of serum concentrations of TNF- alpha and IL-1 beta in test group in a time-dependent manner [P<0.01]. In this study, hydrocortisone was able to reduce the hTLR4/CD14 positive monocytes and its related pro-inflammatory cytokines, thus it can decrease inflammatory responses following PCI
ABSTRACT
In contrast to conventional on-pump coronary artery bypass grafting [CABG] surgery only mild increase of parameters of oxidative stress is reported during and after off-pump coronary artery bypass grafting. In this study, we attempted to determine the role of off-pump CABG in the myocardial and systemic inflammatory responses. One hundred patients who underwent elective CABG were divided to three groups: I] patients underwent off- pump CABG or 2] on-pump CABG surgery with controlled reperfusion and 3] on-pump CABG with noncontrolled reperfusion. We took patients systemic venous blood samples for the measurement of serum level malondialde hyde [MDA], Troponin [cTnI] and total antioxidant [TAC] and blood level superoxiddismotas [SOD], before and after Ischemia and reperfusion. Mean values of decrease left ventricular ejection fraction [LVEF] after surgery in patients group 3 were higher than patients group2 and also [LVEF] in patients group2 were higher than patients group l [P
ABSTRACT
To compare the serum level of MDA [malondialdehyde], antioxidant vitamins, lipoproteins and anthropometry parameters, of 40 patients with coronary artery disease [CAD] and at least one vessel stenosis more than 70% and 40 healthy persons [control group] with mean age of 53.7 +/- 9.45 years were selected for this study. All patients and the healthy controls were evaluated in aspects of anthropometry parameters including weight, height, Basal Metabolic Index [BMI] and waist to hip ratio, angiographic status of coronary arteries [only in patient group], serum levels of triglyceride, cholesterol, LDL-C, HDL2, HDL3, MDA and vitamins E and C. Results showed that BMI and waist to hip ratio in patients were significantly elevated in control group [p<0.01] whereas serum levels of antioxidant vitamins were lower [p<0.001]. Serum levels of MDA in the patients were increased significantly in comparison with the control group [p<0.001]. LDL/HDL ratios were higher and Vitamin E/Cholesterol ratios were lower of patients compared to the control group. These results suggest that decrease of antioxidant vitamins and increase of MDA serum levels play important roles in the pathogenesis of CAD